| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McAuley, Nadia A (10)
|
MRAGMV |
33.32 |
33.19 |
-0.13 |
| 2 |
James, Grace A (10)
|
MAUN |
35.21 |
33.73 |
-1.48 |
| 3 |
Santora, Sofia E (10)
|
MRMWDA |
33.98 |
34.64 |
0.66 |
| 4 |
Grob, Riley C (9)
|
MRNYSA |
38.34 |
35.49 |
-2.85 |
| 5 |
Shaw, Jillian G (9)
|
MRAGMV |
39.62 |
36.95 |
-2.67 |
| 6 |
Bruno, Madison T (10)
|
MRWSSC |
38.38 |
37.13 |
-1.25 |
| 7 |
Zhang, Alisha (10)
|
MRPAC |
42.29 |
37.69 |
-4.60 |
| 8 |
Soperanez, Isabel (10)
|
MRMWDA |
45.99 |
38.82 |
-7.17 |
| 9 |
Coyle, Caitlyn G (9)
|
MRMWDA |
38.82 |
39.66 |
0.84 |
| 10 |
Dwyer, Chloe L (9)
|
MRWSSC |
42.28 |
42.78 |
0.50 |
| 11 |
O'Brien, Abigail C (10)
|
MRAGMV |
44.40 |
44.74 |
0.34 |
| 12 |
Mulqueen, Aylee C (9)
|
MRMWDA |
48.34 |
46.16 |
-2.18 |
| 13 |
Ortiz, Nicole J (10)
|
MRWSSC |
53.02 |
46.91 |
-6.11 |
| 14 |
Lozza, Liliana M (10)
|
MRWSSC |
49.02 |
46.94 |
-2.08 |
| 15 |
Zellner-Portela, Natasha N (10)
|
MRNYSA |
47.42 |
47.08 |
-0.34 |
| 16 |
Sarnicki, Madelyn N (9)
|
MRAGMV |
55.03 |
52.76 |
-2.27 |
| 17 |
Stefanelli, Isabella L (10)
|
MAUN |
48.84 |
53.18 |
4.34 |
| 18 |
Fortune, Alexis B (9)
|
MRWSSC |
58.02 |
55.75 |
-2.27 |